Inhibition of passive cutaneous anaphylaxis-associated scratching behavior by mu-opioid receptor antagonists in ICR mice

Int Arch Allergy Immunol. 2000 Dec;123(4):365-8. doi: 10.1159/000053650.

Abstract

Background: Itching in humans is attenuated by mu-opioid receptor antagonists. ICR mice display increases in scratching behavior upon induction of IgE-mediated passive cutaneous anaphylaxis (PCA), or intradermal injection of compound 48/80 or histamine.

Methods: Cutaneous reactions were induced in ICR mice by IgE-mediated PCA, compound 48/80 and histamine, and the scratching behavior associated with the cutaneous reactions was evaluated.

Results: Naloxone and nalmefene reduced the incidence of scratching behavior associated with PCA. Naloxone also inhibited the induction of scratching behavior caused by compound 48/80 and histamine. Naloxone did not affect the increase in vascular permeability caused by PCA and injection of compound 48/80.

Conclusion: Scratching behavior in mice may be induced by a sensation or a mechanism similar to itching in humans and should become a useful model for examining itching in humans.

MeSH terms

  • Animals
  • Behavior, Animal / drug effects
  • Behavior, Animal / physiology
  • Capillary Permeability / drug effects
  • Female
  • Histamine / pharmacology
  • Humans
  • Mice
  • Mice, Inbred ICR
  • Naloxone / pharmacology*
  • Naltrexone / analogs & derivatives*
  • Naltrexone / pharmacology*
  • Narcotic Antagonists / pharmacology
  • Passive Cutaneous Anaphylaxis / drug effects*
  • Passive Cutaneous Anaphylaxis / immunology
  • Passive Cutaneous Anaphylaxis / physiology
  • Pruritus / drug therapy*
  • Pruritus / immunology
  • Pruritus / physiopathology
  • Receptors, Opioid, mu / antagonists & inhibitors*
  • p-Methoxy-N-methylphenethylamine / pharmacology

Substances

  • Narcotic Antagonists
  • Receptors, Opioid, mu
  • Naloxone
  • p-Methoxy-N-methylphenethylamine
  • Naltrexone
  • Histamine
  • nalmefene