An algorithm combining discrete and continuous methods for optical mapping

J Comput Biol. 2000;7(5):745-60. doi: 10.1089/106652701446189.

Abstract

Optical mapping is a novel technique for generating the restriction map of a DNA molecule by observing many single, partially digested copies of it, using fluorescence microscopy. The real-life problem is complicated by numerous factors: false positive and false negative cut observations, inaccurate location measurements, unknown orientations, and faulty molecules. We present an algorithm for solving the real-life problem. The algorithm combines continuous optimization and combinatorial algorithms applied to a nonuniform discretization of the data. We present encouraging results on real experimental data and on simulated data.

MeSH terms

  • Algorithms*
  • Computer Simulation
  • DNA / genetics
  • Microscopy, Fluorescence
  • Optics and Photonics
  • Restriction Mapping / methods*
  • Restriction Mapping / statistics & numerical data

Substances

  • DNA