Nickel-resistance-based minitransposons: new tools for genetic manipulation of environmental bacteria

S Taghavi; H Delanghe; C Lodewyckx; M Mergeay; D van der Lelie

doi:10.1128/AEM.67.2.1015-1019.2001

Nickel-resistance-based minitransposons: new tools for genetic manipulation of environmental bacteria

Appl Environ Microbiol. 2001 Feb;67(2):1015-9. doi: 10.1128/AEM.67.2.1015-1019.2001.

Authors

S Taghavi¹, H Delanghe, C Lodewyckx, M Mergeay, D van der Lelie

Affiliation

¹ Vlaamse Instelling voor Technologisch Onderzoek (Vito), Environmental Technology Centre, Boeretang 200, 2400 Mol, Belgium.

Abstract

The ncc and nre nickel resistance determinants from Ralstonia eutropha-like strain 31A were used to construct mini-Tn5 transposons. Broad host expression of nickel resistance was observed for the nre minitransposons in members of the alpha, beta, and gamma subclasses of the Proteobacteria, while the ncc minitransposons expressed nickel resistance only in R. eutropha-like strains.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Conjugation, Genetic*
Cupriavidus necator / drug effects*
Cupriavidus necator / genetics
DNA Transposable Elements*
Drug Resistance, Microbial / genetics
Environmental Microbiology*
Nickel / pharmacology*
Proteobacteria / drug effects*
Proteobacteria / genetics

Substances

DNA Transposable Elements
Nickel