Abstract
It has been widely assumed that T cells from TCR-transgenic (Tg) mice better represent the behavior of T cells from normal mice than do in vitro cultures of T cell clones. We have found that autoreactive T cells arising in the presumably more physiological environment of the BDC-2.5 TCR-Tg mouse, despite being apparently "naive" in surface phenotype, are highly activated functionally and do not resemble CD4(+) T cells from a spontaneously diabetic nonobese diabetic (NOD) mouse or the NOD-derived, diabetogenic CD4(+) T cell clone of origin, BDC-2.5. Our results suggest that autoreactive T cells cloned from the spontaneously diabetic NOD mouse more closely resemble effector T cells arising during the natural disease process.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adoptive Transfer
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Aging / genetics
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Aging / immunology
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Animals
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Antibodies, Monoclonal / administration & dosage
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Antigens, Differentiation, T-Lymphocyte / biosynthesis
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Antigens, Differentiation, T-Lymphocyte / genetics
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Autoantigens / genetics*
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / metabolism
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Clone Cells
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Cytokines / biosynthesis
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Cytotoxicity, Immunologic / genetics
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Diabetes Mellitus, Type 1 / genetics
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Diabetes Mellitus, Type 1 / immunology
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Epitopes, T-Lymphocyte / genetics*
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Epitopes, T-Lymphocyte / immunology
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Female
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Immunophenotyping
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Injections, Intravenous
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Integrins / biosynthesis
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Integrins / genetics
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Interphase / genetics
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Interphase / immunology
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L-Selectin / immunology
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Leukemia L1210
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Lymphocyte Activation / genetics
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Membrane Glycoproteins / biosynthesis
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Mice
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Mice, Inbred BALB C
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Mice, Inbred NOD
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Mice, SCID
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Mice, Transgenic
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Perforin
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Phosphorylation
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Phosphotyrosine / metabolism
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Pore Forming Cytotoxic Proteins
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Prediabetic State / genetics
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Prediabetic State / immunology
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Receptors, Antigen, T-Cell / genetics*
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Receptors, Antigen, T-Cell / immunology
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Spleen / immunology
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Spleen / metabolism
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Spleen / pathology
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Spleen / transplantation
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T-Lymphocyte Subsets / immunology*
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T-Lymphocyte Subsets / metabolism*
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T-Lymphocyte Subsets / transplantation
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Thymus Gland / immunology
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Thymus Gland / metabolism
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Thymus Gland / pathology
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Thymus Gland / transplantation
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Transfection
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Transgenes / immunology*
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fas Receptor / genetics
Substances
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Antibodies, Monoclonal
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Antigens, Differentiation, T-Lymphocyte
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Autoantigens
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Cytokines
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Epitopes, T-Lymphocyte
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Integrins
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Membrane Glycoproteins
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Pore Forming Cytotoxic Proteins
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Receptors, Antigen, T-Cell
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fas Receptor
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integrin alpha4beta7
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Perforin
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L-Selectin
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Phosphotyrosine