One-substrate transketolase-catalyzed reaction

Biochem Biophys Res Commun. 2001 Jan 26;280(3):845-7. doi: 10.1006/bbrc.2000.4199.

Abstract

Apart from catalyzing the common two-substrate reaction with ketose as donor substrate and aldose as acceptor substrate, transketolase is also able to catalyze a one-substrate reaction utilizing only ketose (xylulose 5-phosphate) as substrate. The products of this one-substrate reaction were glyceraldehyde 3-phosphate and erythrulose. No free glycolaldehyde (a product of xylulose 5-phosphate splitting in the transketolase reaction) was revealed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Circular Dichroism
  • Glyceraldehyde 3-Phosphate / biosynthesis
  • Kinetics
  • Pentosephosphates / metabolism
  • Saccharomyces cerevisiae / enzymology
  • Substrate Specificity
  • Tetroses / biosynthesis
  • Transketolase / metabolism*

Substances

  • Pentosephosphates
  • Tetroses
  • Glyceraldehyde 3-Phosphate
  • erythrulose
  • xylulose-5-phosphate
  • Transketolase