Abstract
In the screening for cisplatin (CDDP)-resistance related genes by a mRNA differential display method, we detected some increased bands in CDDP resistant ovarian tumor cell line 2008/C13*5.25. One of them, named DD9, was a positive fragment on Northern blot analysis. We cloned it as a full length cDNA by 5'RACE and found a novel gene, CRR9 (Cisplatin Resistance Related gene 9). The CRR9 gene was transcribed into a 2.0 kb mRNA, encoding 512 amino acids. The putative protein had transmembrane-like domains and well conserved on C terminus with human CLPTM1 and the homologs found in Drosophila and C. elegans. Transfection assay showed that the CDDP-sensitive strain 2008 with CRR9 was more sensitive to CDDP, indicating that CRR9 was not associated with the CDDP-resistance, but the CDDP-induced apoptosis.
Copyright 2001 Academic Press.
MeSH terms
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Amino Acid Sequence
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Antineoplastic Agents / pharmacology*
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Apoptosis*
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Base Sequence
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Blotting, Northern
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Cisplatin / pharmacology*
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Cloning, Molecular
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DNA, Complementary / metabolism
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Dose-Response Relationship, Drug
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Drug Resistance, Neoplasm*
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Female
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Gene Expression Profiling
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Humans
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Neoplasm Proteins*
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Oligonucleotides, Antisense
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Ovarian Neoplasms / metabolism*
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Ovarian Neoplasms / pathology*
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Protein Structure, Tertiary
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Transcription, Genetic
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Transfection
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Tumor Cells, Cultured
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Up-Regulation*
Substances
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Antineoplastic Agents
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CLPTM1L protein, human
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DNA, Complementary
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Membrane Proteins
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Neoplasm Proteins
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Oligonucleotides, Antisense
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Proto-Oncogene Proteins c-bcl-2
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RNA, Messenger
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Cisplatin