Variability of human immunodeficiency virus type 2 (hiv-2) infecting patients living in france

Virology. 2001 Feb 1;280(1):19-30. doi: 10.1006/viro.2000.0685.

Abstract

To determine the prevalence of human immunodeficiency virus type 2 (HIV-2) subtypes circulating in France and to identify possible relationships between these subtypes and pathogenesis, we studied 33 HIV-2-infected patients living in France. HIV-2 DNA was directly amplified from peripheral blood mononuclear cells by nested PCR with specific HIV-2 env primers, and the env gene was sequenced. The serological consequences of antigenic variability were studied by using a panel of peptides and by Western blotting. Phylogenetic analysis classified the 33 HIV-2 strains as subtype A (n = 23) or B (n = 10). There were no significant clinical or epidemiological differences between patients infected with either of these two subtypes. There was some evidence for geographical clustering. Subtype A strains from patients originating from the Cape Verde Islands and Guinea Bissau clustered together. The majority of patients infected with subtype B strains originated from the Ivory Coast or Mali. Strains from patients originating in Mali also clustered in subtype A but distinctly from the Cape Verde or Guinea Bissau strains. The subtype B strains showed greater diversity and included some highly divergent strains relative to those previously characterized. The V3 loop of HIV-2 subtypes A and B was found to be quite conserved in comparison with HIV-1. A strong HIV-2 subtype B serological cross-reactivity was found on HIV-1 env antigen by Western blot mostly in the gp41 transmembrane glycoprotein. This could partly explain the double HIV-1 and HIV-2 reactive profiles found in countries where HIV-2 subtype B is prevalent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Amino Acids / analysis
  • Base Sequence
  • Binding Sites
  • Blotting, Western / methods
  • DNA, Viral
  • Epitope Mapping / methods
  • Female
  • France / epidemiology
  • Gene Products, env / genetics
  • Genetic Variation*
  • HIV Antibodies / blood
  • HIV Antibodies / immunology
  • HIV Envelope Protein gp120 / genetics
  • HIV Envelope Protein gp120 / immunology
  • HIV Envelope Protein gp160 / immunology
  • HIV Envelope Protein gp41 / immunology
  • HIV Infections / blood
  • HIV Infections / epidemiology
  • HIV Infections / virology*
  • HIV-2 / classification
  • HIV-2 / genetics*
  • Humans
  • Immunodominant Epitopes / immunology
  • Infant
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Peptide Fragments / genetics
  • Peptide Fragments / immunology
  • Peptides / immunology
  • Phylogeny
  • Polymerase Chain Reaction / methods
  • Prevalence
  • Sequence Homology, Amino Acid

Substances

  • Amino Acids
  • DNA, Viral
  • Gene Products, env
  • HIV Antibodies
  • HIV Envelope Protein gp120
  • HIV Envelope Protein gp160
  • HIV Envelope Protein gp41
  • HIV envelope protein gp120 (305-321)
  • Immunodominant Epitopes
  • Peptide Fragments
  • Peptides

Associated data

  • GENBANK/AF170029
  • GENBANK/AF170030
  • GENBANK/AF170031
  • GENBANK/AF170032
  • GENBANK/AF170033
  • GENBANK/AF170034
  • GENBANK/AF170035
  • GENBANK/AF170036
  • GENBANK/AF170037
  • GENBANK/AF170038
  • GENBANK/AF170039
  • GENBANK/AF170040
  • GENBANK/AF170041
  • GENBANK/AF170042
  • GENBANK/AF170043
  • GENBANK/AF170044
  • GENBANK/AF170045
  • GENBANK/AF170046
  • GENBANK/AF170047
  • GENBANK/AF170048
  • GENBANK/AF170049
  • GENBANK/AF170050
  • GENBANK/AF170051
  • GENBANK/AF170052
  • GENBANK/AF170053
  • GENBANK/AF170054
  • GENBANK/AF170055
  • GENBANK/AF170056
  • GENBANK/AF170057
  • GENBANK/AF170058
  • GENBANK/AF170059
  • GENBANK/AF170060
  • GENBANK/AF170061