Abstract
Retinal levels of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF), an angiogenic inhibitor, were measured and correlated with the ischemia-induced retinal neovascularization in rats. The retinas with neovascularization showed a 5-fold increase in VEGF while 2-fold decrease in PEDF, compared to the age-matched controls, resulting in an increased VEGF/PEDF ratio. The time course of the VEGF/PEDF ratio change correlated with the progression of retinal neovascularization. Changes in the VEGF and PEDF mRNAs preceded their protein level changes. These results suggest that an unbalance between angiogenic stimulators and inhibitors may contribute to retinal neovascularization.
MeSH terms
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Animals
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Animals, Newborn
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Blotting, Northern
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Blotting, Western
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Disease Models, Animal
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Endothelial Growth Factors / genetics*
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Endothelial Growth Factors / metabolism
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Eye Proteins*
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Gene Expression Regulation
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Hyperoxia / physiopathology
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Ischemia / physiopathology*
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Lymphokines / genetics*
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Lymphokines / metabolism
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Neovascularization, Pathologic*
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Nerve Growth Factors*
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Proteins / genetics*
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Proteins / metabolism
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RNA / genetics
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RNA / metabolism
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Rats
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Rats, Inbred BN
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Retina / metabolism
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Retina / pathology*
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Retinal Diseases / genetics
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Retinal Diseases / metabolism
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Retinal Diseases / pathology
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Serpins / genetics*
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Serpins / metabolism
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Time Factors
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
Substances
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Endothelial Growth Factors
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Eye Proteins
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Lymphokines
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Nerve Growth Factors
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Proteins
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Serpins
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
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pigment epithelium-derived factor
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RNA