Isolation of human anti-idiotypes broadly cross reactive with anti-dsDNA antibodies from patients with Systemic lupus erythematosus

Scand J Immunol. 2001 Feb;53(2):192-7. doi: 10.1046/j.1365-3083.2001.00858.x.

Abstract

Antibodies to double stranded (ds)DNA play a central role in clinical diagnosis and disease expression in Systemic lupus erythematosus (SLE). This paper describes the isolation of anti-idiotype reagents (anti/antidsDNA) from four SLE sera and the demonstration of broad and quantitatively similar cross reactivity to both polyclonal and monoclonal anti-dsDNA antibodies isolated from SLE patients. Seven affinity-purified polyclonal and three monoclonal human anti-dsDNA preparations reacted preferentially with anti-idiotype F(ab')(2) coated plates compared to normal immunoglobulin (Ig)G F(ab')(2) coated plates in ELISA. In contrast, autoantibodies of other specificities (anti-Ro/SSA, anti-La/SSB, and anti-U(1)RNP) reacted equally with anti/anti-dsDNA F(ab')(2) and normal IgG F(ab')(2) coated plates. Such anti-idiotypic antibodies could play a significant role in the regulation of anti-dsDNA antibody levels in SLE.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Anti-Idiotypic / blood
  • Antibodies, Anti-Idiotypic / immunology
  • Antibodies, Anti-Idiotypic / isolation & purification*
  • Antibodies, Antinuclear / immunology*
  • Antibodies, Monoclonal / immunology
  • Antibody Specificity
  • Antigen-Antibody Reactions
  • Autoantigens / immunology*
  • Autoimmune Diseases / blood
  • Autoimmune Diseases / immunology*
  • Chromatography, Affinity
  • Cross Reactions
  • DNA / immunology*
  • Humans
  • Immunoglobulin Fab Fragments / immunology
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Immunoglobulin G / isolation & purification*
  • Lupus Erythematosus, Systemic / blood
  • Lupus Erythematosus, Systemic / immunology*
  • RNA, Small Cytoplasmic*
  • Ribonucleoprotein, U1 Small Nuclear / immunology
  • Ribonucleoproteins / immunology
  • SS-B Antigen

Substances

  • Antibodies, Anti-Idiotypic
  • Antibodies, Antinuclear
  • Antibodies, Monoclonal
  • Autoantigens
  • Immunoglobulin Fab Fragments
  • Immunoglobulin G
  • RNA, Small Cytoplasmic
  • RO60 protein, human
  • Ribonucleoprotein, U1 Small Nuclear
  • Ribonucleoproteins
  • SS-A antibodies
  • SS-A antigen
  • DNA