Abstract
5-Hexadecenoic acid can be used to produce a semichemical method to control the disease-carrying mosquito Culex quinquefasciatus. This unusual fatty acid is produced in the seed of Kochia scoparia. We have isolated two acyl-acyl carrier protein desaturases from this species and expressed them in Escherichia coli to facilitate functional characterisation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cloning, Molecular
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Culex
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Escherichia coli
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Fatty Acids / metabolism*
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Gene Amplification
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Isoenzymes / genetics
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Isoenzymes / isolation & purification
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Isoenzymes / metabolism
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Magnoliopsida / enzymology*
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Magnoliopsida / genetics
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Mixed Function Oxygenases / genetics
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Mixed Function Oxygenases / isolation & purification*
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Mixed Function Oxygenases / metabolism*
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Palmitic Acids / metabolism*
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Pest Control, Biological
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Seeds / enzymology
Substances
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Fatty Acids
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Isoenzymes
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Palmitic Acids
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Recombinant Proteins
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hexadecenoic acid
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Mixed Function Oxygenases
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acyl-(acyl-carrier-protein)desaturase