Abstract
Aquilegia vulgaris seed oil contains high levels of the rare fatty acid columbinic acid (18:3 Delta(5,9,12)), which is unusual in having the double bond at the Delta(5) carbon in the trans configuration. Columbinic acid was found to be a seed-specific fatty acid not only present in the storage oil but also in membrane lipids. Several putative gene fragments have been isolated from plant RNA with sequences similar to previously characterized 'front-end' desaturases. Functional characterization of the Aquilegia cDNA is underway.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding Sites
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Cytochromes b5 / metabolism
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Fatty Acid Desaturases / genetics
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Fatty Acid Desaturases / metabolism*
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Linolenic Acids / analysis
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Linolenic Acids / metabolism
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Lipids / chemistry*
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Membrane Lipids / chemistry
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Open Reading Frames
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Plant Oils / chemistry
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Plants / enzymology*
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Plants / genetics
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Seeds / chemistry
Substances
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Linolenic Acids
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Lipids
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Membrane Lipids
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Plant Oils
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5,9,12-octadecatrienoic acid
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Cytochromes b5
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Fatty Acid Desaturases