Abstract
CD4+ memory T cells (Tm) from rheumatoid arthritis peripheral blood (RAPB) or peripheral blood from normal donors produced IL-2, whereas fewer cells secreted IFN-gamma or IL-4 after a brief stimulation. RAPB Tm contained significantly more IFN-gamma producers than normal cells. Many rheumatoid arthritis (RA) synovial Tm produced IFN-gamma alone (40%) and fewer cells produced IL-2 or IL-4. An in vitro model was employed to generate polarized T-helper (Th) effectors. Normal and RAPB Tm differentiated into both IFN-gamma- and IL-4-producing effectors. RA synovial fluid (RASF) Tm demonstrated defective responsiveness, exhibiting diminished differentiation of IL-4 effectors, whereas RA synovial tissue (RAST) Tm exhibited defective generation of IFN-gamma and IL-4 producers.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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Aged
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Arthritis, Rheumatoid / immunology*
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Arthritis, Rheumatoid / pathology
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CD4-Positive T-Lymphocytes / immunology*
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CD4-Positive T-Lymphocytes / metabolism
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CD4-Positive T-Lymphocytes / pathology
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Cell Differentiation / immunology
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Cells, Cultured
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Humans
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Immune Sera / pharmacology
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Interferon-gamma / biosynthesis
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Interleukin-2 / biosynthesis
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Interleukin-4 / biosynthesis*
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Interleukin-4 / metabolism
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Leukocyte Common Antigens / biosynthesis
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Middle Aged
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Signal Transduction / immunology
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Synovial Membrane / immunology*
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Synovial Membrane / pathology
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T-Lymphocyte Subsets / immunology
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T-Lymphocyte Subsets / metabolism
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T-Lymphocyte Subsets / pathology
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Th2 Cells / immunology*
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Th2 Cells / metabolism
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Th2 Cells / pathology
Substances
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Immune Sera
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Interleukin-2
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Interleukin-4
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Interferon-gamma
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Leukocyte Common Antigens