Molecular epidemiology of human astrovirus diarrhea among children from a periurban community of Mexico City

J Infect Dis. 2001 Mar 1;183(5):681-6. doi: 10.1086/318825. Epub 2001 Jan 31.

Abstract

Human astroviruses (HAstVs) were detected in 23 stool samples from 365 diarrhea episodes among 214 children (<18 months old) prospectively monitored for diarrhea in Mexico City. Stool samples were tested by EIA and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. EIA was less sensitive (74%) and equally specific, compared with RT-PCR analysis using type-common primers for HAstV detection. Of 31 HAstV isolates, EIA typed 18 (69%) of 26 EIA-positive samples, and RT-PCR analysis typed 26 (84%) of 31 RT-PCR-positive samples. Phylogenetic analysis of the 3' end of the capsid region (363 nucleotides) confirmed the type assignment by EIA and RT-PCR analysis and determined the type for 5 previously untyped samples. Six HAstV antigenic types cocirculated in the community: HAstV-2 (42%), HAstV-4 (23%), HAstV-3 (13%), HAstV-1 (10%), HAstV-5 (6%), and HAstV-7 (6%). RT-PCR and sequence analysis provided more detailed epidemiology of HAstV in the community than did antigenic detection methods.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Astroviridae Infections / epidemiology*
  • Astroviridae Infections / virology
  • Base Sequence
  • Caco-2 Cells
  • DNA Primers
  • Diarrhea, Infantile / epidemiology*
  • Diarrhea, Infantile / virology
  • Disease Outbreaks
  • Feces / virology
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Infant
  • Infant, Newborn
  • Male
  • Mamastrovirus / classification*
  • Mamastrovirus / genetics
  • Mamastrovirus / isolation & purification
  • Mexico / epidemiology
  • Molecular Epidemiology
  • Molecular Sequence Data
  • Phylogeny
  • Prospective Studies
  • RNA, Viral / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Seroepidemiologic Studies
  • Urban Population / statistics & numerical data

Substances

  • DNA Primers
  • RNA, Viral

Associated data

  • GENBANK/AF248738
  • GENBANK/AF257221
  • GENBANK/AF257222
  • GENBANK/AF257223
  • GENBANK/AF257224
  • GENBANK/AF257225
  • GENBANK/AF257226
  • GENBANK/AF257227
  • GENBANK/AF257228
  • GENBANK/AF257229