Molecular cloning of a novel human gene (SIRP-B2) which encodes a new member of the SIRP/SHPS-1 protein family

J Hum Genet. 2000;45(6):378-82. doi: 10.1007/s100380070013.

Abstract

A full-length cDNA encoding a novel protein was isolated and sequenced from a human placental cDNA library. This cDNA consists of 1,735 base pairs and has a predicted open reading frame (ORF) encoding 354 amino acids. It possesses a putative signal sequence, a long extracellular domain, a transmembrane region, a short intracellular domain, and no catalytic domain, which is highly homologous to signal-regulatory protein (SIRP)-beta suggesting that it seems to be a new member of the SIRP family. Polymerase chain reaction (PCR)-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 20p13 near the marker D20S906.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Differentiation*
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 20 / genetics
  • Cloning, Molecular*
  • Humans
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics*
  • Molecular Sequence Data
  • Neural Cell Adhesion Molecule L1*
  • Neural Cell Adhesion Molecules / chemistry
  • Neural Cell Adhesion Molecules / genetics*
  • Receptors, Immunologic*
  • Sequence Analysis, DNA

Substances

  • Antigens, Differentiation
  • Membrane Glycoproteins
  • Neural Cell Adhesion Molecule L1
  • Neural Cell Adhesion Molecules
  • Receptors, Immunologic
  • SIRPB2 protein, human

Associated data

  • GENBANK/AB042624