Detection of the Cryptosporidium parvum "human" genotype in a dugong (Dugong dugon)

J Parasitol. 2000 Dec;86(6):1352-4. doi: 10.1645/0022-3395(2000)086[1352:DOTCPH]2.0.CO;2.

Abstract

The Cryptosporidium "human" genotype was identified in a paraffin-embedded tissue section from a dugong (Dugong dugon) by 2 independent laboratories. DNA sequencing and polymerase chain reaction/restriction fragment length polymorphism analysis of the 18S ribosomal RNA gene and the acetyl CoA synthethase gene clearly identified the genotype as that of the Cryptosporidium variant that infects humans. This is the first report of the human Cryptosporidium genotype in a nonprimate host.

Publication types

  • Case Reports

MeSH terms

  • Acetate-CoA Ligase / genetics
  • Animals
  • Cryptosporidiosis / parasitology
  • Cryptosporidiosis / veterinary*
  • Cryptosporidium parvum / classification*
  • Cryptosporidium parvum / genetics
  • Cryptosporidium parvum / isolation & purification
  • DNA, Protozoan / chemistry
  • DNA, Ribosomal / chemistry
  • Dugong / parasitology*
  • Genotype
  • Humans
  • Opportunistic Infections / parasitology
  • Opportunistic Infections / veterinary
  • Polymerase Chain Reaction / veterinary
  • Polymorphism, Restriction Fragment Length
  • Queensland
  • RNA, Ribosomal, 18S / genetics
  • Sequence Alignment / veterinary
  • Zoonoses

Substances

  • DNA, Protozoan
  • DNA, Ribosomal
  • RNA, Ribosomal, 18S
  • Acetate-CoA Ligase