Abstract
Screening of the Merck sample collection for compounds with CCR5 receptor binding afforded (2S)-2-(3,4-dichlorophenyl)-1-[N-(methyl)-N-(phenylsulfonyl)amino]-4-[spiro(2,3-dihydrobenzthiophene-3,4'-piperidin-1'-yl)]butane S-oxide (4) as a potent lead structure having an IC50 binding affinity of 35 nM. Herein, we describe the discovery of this lead structure and our initial structure activity relationship studies directed toward the requirement for and optimization of the 1-amino fragment.
MeSH terms
-
Animals
-
Anti-HIV Agents / chemical synthesis*
-
Anti-HIV Agents / chemistry
-
Anti-HIV Agents / metabolism
-
CCR5 Receptor Antagonists*
-
CHO Cells
-
Chemokine CCL4
-
Combinatorial Chemistry Techniques
-
Cricetinae
-
Humans
-
Inhibitory Concentration 50
-
Macrophage Inflammatory Proteins / metabolism
-
Piperidines / chemical synthesis
-
Piperidines / chemistry
-
Piperidines / metabolism
-
Protein Binding
-
Receptors, CCR5 / genetics
-
Receptors, CCR5 / metabolism
-
Structure-Activity Relationship
-
Transfection
Substances
-
Anti-HIV Agents
-
CCR5 Receptor Antagonists
-
Chemokine CCL4
-
Macrophage Inflammatory Proteins
-
Piperidines
-
Receptors, CCR5