Abstract
(2S)-2-(3,4-Dichlorophenyl)-1-[N-(methyl)-N-(phenylsulfonyl)amino]-4-[spiro(2,3-dihydrobenzthiophene-3,4'-piperidin-1'-yl)]butane S-oxide (3) has been identified as a potent CCR5 antagonist lead structure having an IC50 = 35 nM. Herein, we describe the structure-activity relationship studies directed toward the requirement for and optimization of the C-2 phenyl fragment. The phenyl was found to be important for CCR5 antagonism and substitution was limited to small moieties at the 3-position (13 and 16: X= H, 3-F, 3-Cl, 3-Me).
MeSH terms
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Animals
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Anti-HIV Agents / chemical synthesis*
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Anti-HIV Agents / chemistry
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Anti-HIV Agents / metabolism
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Butanes / chemical synthesis
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Butanes / chemistry
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Butanes / metabolism
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Butylamines / chemical synthesis
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Butylamines / chemistry
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Butylamines / metabolism
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CCR5 Receptor Antagonists*
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CHO Cells
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Chemokine CCL4
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Combinatorial Chemistry Techniques
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Cricetinae
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Humans
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Inhibitory Concentration 50
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Macrophage Inflammatory Proteins / metabolism
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Piperidines / chemical synthesis
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Piperidines / chemistry
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Piperidines / metabolism
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Protein Binding
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Receptors, CCR5 / genetics
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Receptors, CCR5 / metabolism
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Structure-Activity Relationship
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Sulfonamides / chemical synthesis
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Sulfonamides / chemistry
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Sulfonamides / metabolism
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Transfection
Substances
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Anti-HIV Agents
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Butanes
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Butylamines
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CCR5 Receptor Antagonists
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Chemokine CCL4
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Macrophage Inflammatory Proteins
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Piperidines
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Receptors, CCR5
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Sulfonamides