Abstract
Mice that had received adoptive transfer of DO11.10 TCR transgenic T cells polarized toward a Th1 or a Th2 phenotype were challenged with Ag-coated beads or with recombinant Mycobacterium tuberculosis expressing the OVA determinant. The resulting bead-induced pulmonary granulomas reflected the phenotype of the adoptively transferred T cells, with the Th2 cells promoting a fibrotic reaction. Mice receiving Th1 cells mounted an epitope-specific protective response to challenge with recombinant M. tuberculosis. Th2 recipients were characterized by enhanced weight loss and lung fibrosis during acute high-dose infection. The combination of TCR transgenic T cells and epitope-tagged mycobacteria provides a novel experimental model for investigation of the pathogenesis of tuberculosis.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adoptive Transfer
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Animals
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Antigens / administration & dosage
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Antigens / immunology
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Cells, Cultured
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Cytokines / biosynthesis
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Disease Models, Animal
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Female
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Granuloma, Respiratory Tract / etiology
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Granuloma, Respiratory Tract / immunology*
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Granuloma, Respiratory Tract / pathology*
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Granuloma, Respiratory Tract / prevention & control
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Injections, Intravenous
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Lymphocyte Transfusion
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Mice
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Mice, Inbred BALB C
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Mice, Transgenic
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Microspheres
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Mycobacterium tuberculosis / immunology
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Ovalbumin / administration & dosage
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Ovalbumin / immunology
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Spleen / cytology
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Spleen / immunology
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Spleen / transplantation
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Th1 Cells / immunology*
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Th1 Cells / metabolism
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Th1 Cells / transplantation
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Th2 Cells / immunology*
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Th2 Cells / metabolism
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Th2 Cells / transplantation
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Tuberculosis / etiology
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Tuberculosis / immunology*
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Tuberculosis / pathology*
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Tuberculosis / prevention & control
Substances
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Antigens
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Cytokines
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Ovalbumin