cDNA cloning of the Cry1Aa receptor variants from Bombyx mori and their expression in mammalian cells

S Ikawa; Y Tsuda; T Fukada; K Sugimoto; M Himeno

doi:10.1271/bbb.64.2682

cDNA cloning of the Cry1Aa receptor variants from Bombyx mori and their expression in mammalian cells

Biosci Biotechnol Biochem. 2000 Dec;64(12):2682-5. doi: 10.1271/bbb.64.2682.

Authors

S Ikawa¹, Y Tsuda, T Fukada, K Sugimoto, M Himeno

Affiliation

¹ Department of Applied Biochemistry, Osaka Prefecture University, Sakai, Japan.

PMID: 11210135
DOI: 10.1271/bbb.64.2682

Abstract

We cloned cDNA of three variants of BtR175, a putative Bombyx mori receptor for Bacillus thuringiensis Cry1Aa delta-endotoxin by PCR. These variants were likely to be allelic to BtR175. cDNA of BtR175b, the most distant variant from BtR175, was introduced into mammalian cells. BtR175b protein was expressed in the plasma membrane of the cells and showed binding activity to Cry1Aa.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alternative Splicing
Amino Acid Substitution
Animals
Bacterial Proteins
Bombyx / genetics*
COS Cells
Cell Line
Cloning, Molecular
DNA, Complementary
Genetic Variation
Humans
Insect Proteins*
Mammals
Molecular Sequence Data
Receptors, Cell Surface / genetics*
Receptors, Cell Surface / metabolism*
Recombinant Proteins / genetics
Recombinant Proteins / metabolism

Substances

Bacterial Proteins
Cry toxin receptors
DNA, Complementary
Insect Proteins
Receptors, Cell Surface
Recombinant Proteins

Associated data

GENBANK/AB041508
GENBANK/AB041509
GENBANK/AB041510