Expression and analysis of ARNO and ARNO mutants and their effects on ADP-ribosylation factor (ARF)-mediated actin cytoskeletal rearrangements

Methods Enzymol. 2001:329:256-64. doi: 10.1016/s0076-6879(01)29086-7.

Authors

L C Santy¹, S R Frank, J E Casanova

Affiliation

¹ Department of Cell Biology, University of Virginia, Health Sciences Center, Charlottesville, Virginia 22908, USA.

PMID: 11210542
DOI: 10.1016/s0076-6879(01)29086-7

No abstract available

MeSH terms

ADP-Ribosylation Factors / metabolism*
Actins / metabolism*
Chromatography, Thin Layer
Cytoskeleton / metabolism*
Fluorescent Antibody Technique
Fungal Proteins / chemistry
GTPase-Activating Proteins / chemistry
GTPase-Activating Proteins / genetics
GTPase-Activating Proteins / metabolism*
Guanine Nucleotide Exchange Factors*
Guanosine Triphosphate / metabolism
HeLa Cells
Humans
Mutation / genetics*
Phosphatidylethanolamines
Staining and Labeling
Tissue Fixation
Transfection

Substances

Actins
Fungal Proteins
GTPase-Activating Proteins
Guanine Nucleotide Exchange Factors
Phosphatidylethanolamines
Sec7 guanine nucleotide exchange factors
cytohesin-2
1,2-dielaidoylphosphatidylethanolamine
Guanosine Triphosphate
ADP-Ribosylation Factors