Abstract
A new nucleoside derivative (2) with a butyl spacer between the sugar part and the 2-amino-6-vinylpurine motif has been synthesized. The triplex-forming oligodeoxynucleotide incorporating 2 has achieved strand- and cytidine-selective cross-linking reaction to the G-C target site mediated by triple helix formation. It has been suggested that 2 reacts with a flipping cytidine at the target site.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Pair Mismatch*
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Base Pairing
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Cross-Linking Reagents / chemical synthesis
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Cross-Linking Reagents / chemistry
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Cross-Linking Reagents / metabolism
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Cytidine / chemistry*
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DNA / chemistry*
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DNA / metabolism
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DNA / ultrastructure
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Electrophoresis
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Nucleic Acid Conformation
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Nucleosides / chemical synthesis
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Nucleosides / chemistry
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Nucleosides / metabolism
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Oligodeoxyribonucleotides / chemical synthesis
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Oligodeoxyribonucleotides / chemistry
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Oligodeoxyribonucleotides / metabolism
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Structure-Activity Relationship
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Temperature
Substances
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Cross-Linking Reagents
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Nucleosides
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Oligodeoxyribonucleotides
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Cytidine
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DNA