Abstract
Using UV-vis spectrometrical measurements, equilibrium constants for NO transfer between S-nitroso-N-acetyl-penicillamine (SNAP) and different thiols as well as kinetic data for NO transfer from S-nitroso bovine serum albumin (BSANO) to thiols have been obtained. NO transfer from SNAP to other primary/secondary thiols are thermodynamically favorable, whereas other S-nitrosothiols exhibit similar NO transfer potential. The obtained Gibbs free energy, enthalpy and entropy data indicated that NO transfer reactions from SNAP to four thiols are exothermic with entropy loss. The kinetic behavior of BSANO/RSH transfer can be related to both the acidity of sulfhydryl group and the electronic structure in thiol.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Kinetics
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Mercaptoethanol*
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Nitrosation
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Nitroso Compounds / chemistry
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Nitroso Compounds / metabolism*
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Penicillamine / analogs & derivatives
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Penicillamine / chemistry
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Penicillamine / metabolism
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S-Nitroso-N-Acetylpenicillamine
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S-Nitrosothiols*
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Serum Albumin, Bovine / chemistry
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Serum Albumin, Bovine / metabolism
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Spectrophotometry, Ultraviolet
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Sulfhydryl Compounds / chemistry
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Sulfhydryl Compounds / metabolism*
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Thermodynamics
Substances
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Nitroso Compounds
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S-Nitrosothiols
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S-nitrosoalbumin
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Sulfhydryl Compounds
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Serum Albumin, Bovine
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Mercaptoethanol
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S-nitrosomercaptoethanol
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S-Nitroso-N-Acetylpenicillamine
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Penicillamine