Abstract
Rhodococcus sp. 1BN was isolated from a contaminated site and showed various biodegradative capabilities. Besides naphthalene, strain 1BN degraded medium- (C6) and long-chain alkanes (C16-C28), benzene and toluene, alone or when the hydrocarbons were mixed in equal proportions. The nucleotide sequence of an alk polymerase chain reaction (PCR) fragment revealed a 59% nucleotide homology to the Pseudomonas oleovorans alkB gene. The nar fragments were highly homologous to genes coding for large and small subunits of cis-naphthalene 1,2-dioxygenase (narAa and narAb) and to cis-naphthalene dihydrodiol dehydrogenase (narB) from other rhodococci. The oxidation of indene to cis-(1S,2R)-1,2-dihydroxyindan by toluene-induced cells allows to hypothesize that strain 1BN also carries a toluene dioxygenase-like system.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkanes / metabolism*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Chromatography, High Pressure Liquid
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Cytochrome P-450 CYP4A
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Cytochrome P-450 Enzyme System / genetics
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Cytochrome P-450 Enzyme System / metabolism
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Genes, Bacterial*
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Indenes / metabolism
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Mixed Function Oxygenases / genetics
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Mixed Function Oxygenases / metabolism
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Molecular Sequence Data
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Naphthalenes / metabolism*
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Nitrate Reductase
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Nitrate Reductases / genetics
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Nitrate Reductases / metabolism
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Oxidation-Reduction
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Oxygenases / metabolism
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Protein Subunits
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Pseudomonas / genetics*
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Pseudomonas / metabolism
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Rhodococcus / genetics*
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Rhodococcus / metabolism
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Sequence Homology, Nucleic Acid
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Toluene / metabolism
Substances
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Alkanes
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Bacterial Proteins
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Indenes
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Naphthalenes
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Protein Subunits
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narA protein, Bacillus subtilis
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naphthalene
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Toluene
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indene
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Cytochrome P-450 Enzyme System
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Mixed Function Oxygenases
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Oxygenases
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toluene dioxygenase
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Cytochrome P-450 CYP4A
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Nitrate Reductases
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Nitrate Reductase
Associated data
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GENBANK/AJ401611
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GENBANK/AJ401612
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GENBANK/AJ401613