Abstract
Aim of this work was to investigate the ability of the antibodies against Surfactant proteins (SP) and Thyroid transcription factor 1 (TTF-1) to distinguish primary neoplasms of the lung from metastatic carcinomas to the lung and pleural mesotheliomas. We evaluated the immunohistochemical expression of the antibodies anti SP-A, SP-B, pro SP-C, SP-D, and TTF-1 in a series of 56 primary lung carcinomas, 9 metastatic carcinomas to the lung, 5 pleural mesotheliomas and 8 non-pulmonary carcinomas. Among primary lung neoplasms, only adenocarcinomas immunostained for all SP (specificity = 1; total sensitivity = 0.52). TTF-1 had an excellent specificity (= 1), but a weak sensitivity (= 0.34) in recognizing primary lung carcinomas. TTF-1 was present in lung adenocarcinomas which were negative for SPs; however it failed to distinguish the subtypes. Pleural mesotheliomas, pulmonary metastases and non-pulmonary carcinomas were not immunoreactive for SP-A, SP-B, SP-D, and TTF-1. Pro SP-C was positive also in the adenocarcinomas of the large bowel and in their pulmonary and nodal metastases. These results demonstrate that the combined use of antibodies anti SP-A, SP-B and TTF-1 is the best association in distinguishing primary lung carcinomas from metastatic carcinomas to the lung and pleural mesotheliomas.
MeSH terms
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Adenocarcinoma / chemistry
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Adenocarcinoma / diagnosis
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Adenocarcinoma / secondary
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Biomarkers, Tumor / analysis*
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Carcinoma / chemistry
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Carcinoma / diagnosis*
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Carcinoma / pathology
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Carcinoma / secondary
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Colorectal Neoplasms / chemistry
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Diagnosis, Differential
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Glycoproteins / analysis*
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Humans
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Immunoenzyme Techniques*
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Lung Neoplasms / chemistry
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Lung Neoplasms / diagnosis*
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Lung Neoplasms / pathology
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Lung Neoplasms / secondary
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Lymphatic Metastasis
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Mesothelioma / chemistry
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Mesothelioma / diagnosis*
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Mesothelioma / pathology
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Neoplasm Proteins / analysis*
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Nuclear Proteins / analysis*
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Pleural Neoplasms / chemistry
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Pleural Neoplasms / diagnosis*
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Pleural Neoplasms / pathology
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Pleural Neoplasms / secondary
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Protein Precursors / analysis
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Proteolipids / analysis*
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Pulmonary Surfactant-Associated Protein A
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Pulmonary Surfactant-Associated Protein D
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Pulmonary Surfactant-Associated Proteins
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Pulmonary Surfactants / analysis*
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Thyroid Nuclear Factor 1
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Transcription Factors / analysis*
Substances
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Biomarkers, Tumor
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Glycoproteins
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NKX2-1 protein, human
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Neoplasm Proteins
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Nuclear Proteins
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Protein Precursors
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Proteolipids
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Pulmonary Surfactant-Associated Protein A
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Pulmonary Surfactant-Associated Protein D
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Pulmonary Surfactant-Associated Proteins
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Pulmonary Surfactants
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Thyroid Nuclear Factor 1
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Transcription Factors