Efficacy of treatment with antisense oligonucleotides complementary to immunoglobulin sequences of bcl-2/immunoglobulin fusion transcript in a t(14;18) human lymphoma-scid mouse model

Clin Cancer Res. 2001 Feb;7(2):400-6.

Abstract

In t(14;18)-positive lymphoma cells, bcl-2 is expressed from a fusion mRNA transcript containing the full coding sequence of bcl-2 and 3' immunoglobulin sequences. We reported previously that antisense oligodeoxyribonucleotides directed at the bcl-2 translational start site, as well as those targeted to immunoglobulin sequences 3' of the translocation breakpoint, down-regulate bcl-2 and inhibit growth of the t(14;18)-positive lymphoma line WSU-FSCCL in vitro. We have developed a scid mouse model with this human cell line and demonstrate that antisense oligodeoxyribonucleotides targeted to immunoglobulin c(mu) sequences down-regulate bcl-2 protein expression and induce apoptosis of WSU-FSCCL cells in vivo. This leads to prolonged survival of the mice. Targeting non-oncogenic sequences outside of the breakpoints of fusion transcripts may be a clinically useful therapeutic strategy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Apoptosis / immunology
  • Blotting, Western
  • Chromosomes, Human, Pair 14*
  • Chromosomes, Human, Pair 18*
  • Down-Regulation
  • Female
  • Flow Cytometry
  • Gene Expression
  • Genes, bcl-2*
  • Humans
  • Immunoblotting / methods
  • Immunoglobulin Fragments / genetics*
  • Lymphoma / drug therapy*
  • Mice
  • Mice, SCID
  • Oligonucleotides, Antisense / therapeutic use*
  • Oncogene Proteins, Fusion / genetics*
  • Severe Combined Immunodeficiency / drug therapy*
  • Survival Analysis
  • Tumor Cells, Cultured

Substances

  • Immunoglobulin Fragments
  • Oligonucleotides, Antisense
  • Oncogene Proteins, Fusion