The murine p202 protein, an IFN-inducible modulator of transcription, is activated by the mitogen platelet-derived growth factor

J Interferon Cytokine Res. 2001 Feb;21(2):99-103. doi: 10.1089/107999001750069962.

Abstract

p202 is a murine interferon (IFN)-inducible protein belonging to a cluster of IFN-inducible genes (the 200 family) located in a segment of chromosome 1. It is a nuclear DNA-binding protein that is able to modulate transcription by interacting with a heterogeneous set of transcription factors, including NF-kappaB, (p50/p65), AP-1, c-fos, c-jun, and RB-1. The p202 protein is believed to attenuate cell growth/proliferation, mainly through the activation of IFN-stimulated of gene factor 3 (ISGF3), which binds IFN-stimulated response elements (ISRE) located in the promoters of type I IFN genes. In this report, we show that the p202 gene can also be induced by platelet-derived growth factor (PDGF), a mitogen known to drive G(0)-arrested cells toward reentry into the cell cycle. PDGF transiently enhances the steady-state mRNA level of p202 and increases the p202 protein level independently from IFN signaling, by acting at the transcriptional level on its promoter. The kinetics of p202 induction by PDGF are faster and more transient than those of IFN. These data identify p202 as a member of the IFN-inducible gene family that can be directly regulated by mitogenic stimuli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Interferon Type I / genetics
  • Interferon Type I / pharmacology
  • Intracellular Signaling Peptides and Proteins*
  • Mice
  • Mitogens / pharmacology
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism*
  • Platelet-Derived Growth Factor / pharmacology*
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Transcription, Genetic

Substances

  • Carrier Proteins
  • Ifi202b protein, mouse
  • Interferon Type I
  • Intracellular Signaling Peptides and Proteins
  • Mitogens
  • Nuclear Proteins
  • Phosphoproteins
  • Platelet-Derived Growth Factor
  • RNA, Messenger