Transforming growth factor-beta 1 and insulin-like growth factor-1 expression in ovarian endometriotic cysts: a preliminary study

Int J Mol Med. 2001 Apr;7(4):423-9. doi: 10.3892/ijmm.7.4.423.

Abstract

Increased concentrations of TGF-beta 1 in endometriotic tissue are considered important in the pathophysiology of endometrial diseases since TGF-beta 1 may inhibit natural killer activity and induce angiogenesis and proliferation of endometrial stromal cells. In the present study we report on TGF-beta 1, IGF-1 and their receptor localization, as detected by Northern hybridization and immunohistochemistry, in ovarian endometriotic tissues removed during surgical procedures. We detected comparable expression of IGF-1 and IGF-1 receptor in the stromal and epithelial compartments, thus confirming disregulated expression of the IGF system in ovarian endometriosis. On the contrary, strongly increased TGF-beta 1 steady state level mRNA expression was detected in all endometriotic samples. In addition, we demonstrated weak TGF-beta 1 immunohistochemical expression in the epithelial lining and intense expression in the cellular stroma of ovarian endometriomas, thus suggesting that TGF-beta 1 could have an important role in the maintenance and propagation of the disease. On the basis of these preliminary results we can assume that TGF-beta 1, IGF-1 and their receptors may play an important role in the pathogenesis of endometriosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Blotting, Northern
  • Endometriosis / metabolism*
  • Endometriosis / pathology
  • Endometrium / chemistry
  • Endometrium / metabolism
  • Endometrium / pathology
  • Female
  • Humans
  • Immunohistochemistry
  • Insulin-Like Growth Factor I / biosynthesis*
  • Macrophages / chemistry
  • Macrophages / pathology
  • Ovarian Cysts / metabolism*
  • Ovarian Cysts / pathology
  • Pilot Projects
  • RNA, Neoplasm / biosynthesis
  • Receptor, IGF Type 1 / analysis
  • Receptors, Transforming Growth Factor beta / analysis
  • Stromal Cells / chemistry
  • Stromal Cells / pathology
  • Transforming Growth Factor beta / biosynthesis*
  • Transforming Growth Factor beta1

Substances

  • RNA, Neoplasm
  • Receptors, Transforming Growth Factor beta
  • TGFB1 protein, human
  • Transforming Growth Factor beta
  • Transforming Growth Factor beta1
  • Insulin-Like Growth Factor I
  • Receptor, IGF Type 1