Abstract
The efficient application of biocatalysts requires the availability of suitable enzymes with high activity and stability under process conditions, desired substrate selectivity and high enantioselectivity. However, wild-type enzymes often need to be optimized to fulfill these requirements. Two rather contradictory tools can be used on a molecular level to create tailor-made biocatalysts: directed evolution and rational protein design.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Catalysis
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Directed Molecular Evolution / methods*
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Directed Molecular Evolution / trends
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Enzyme Activation / physiology
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Enzyme Stability / physiology
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Enzymes / metabolism*
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Esterases / genetics
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Gene Library
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Mutagenesis, Site-Directed / genetics
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Protein Engineering / methods*
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Protein Engineering / trends
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Pseudomonas fluorescens / enzymology
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Stereoisomerism
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Substrate Specificity / physiology