Abstract
Strain IMB-1, an aerobic methylotrophic member of the alpha subgroup of the Proteobacteria, can grow with methyl bromide as a sole carbon and energy source. A single cmu gene cluster was identified in IMB-1 that contained six open reading frames: cmuC, cmuA, orf146, paaE, hutI, and partial metF. CmuA from IMB-1 has high sequence homology to the methyltransferase CmuA from Methylobacterium chloromethanicum and Hyphomicrobium chloromethanicum and contains a C-terminal corrinoid-binding motif and an N-terminal methyltransferase motif. However, cmuB, identified in M. chloromethanicum and H. chloromethanicum, was not detected in IMB-1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alphaproteobacteria / genetics
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Alphaproteobacteria / growth & development
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Alphaproteobacteria / metabolism
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Amino Acid Sequence
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Bacterial Proteins*
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Culture Media
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Genes, Bacterial*
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Gram-Negative Bacteria / genetics*
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Gram-Negative Bacteria / growth & development*
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Gram-Negative Bacteria / metabolism
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Hydrocarbons, Brominated / metabolism*
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Methyltransferases / chemistry
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Methyltransferases / genetics*
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Methyltransferases / metabolism*
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Molecular Sequence Data
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Sequence Analysis, DNA
Substances
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Bacterial Proteins
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Culture Media
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Hydrocarbons, Brominated
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methyl bromide
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CmuA protein, Methylobacterium chloromethanicum
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Methyltransferases