Abstract
We report the development of a pseudorabies virus that can be used for retrograde tracing from selected neurons. This virus encodes a green fluorescent protein marker and replicates only in neurons that express the Cre recombinase and in neurons in synaptic contact with the originally infected cells. The virus was injected into the arcuate nucleus of mice that express Cre only in those neurons that express neuropeptide Y or the leptin receptor. Sectioning of the brains revealed that these neurons receive inputs from neurons in other regions of the hypothalamus, as well as the amygdala, cortex, and other brain regions. These data suggest that higher cortical centers modulate leptin signaling in the hypothalamus. This method of neural tracing may prove useful in studies of other complex neural circuits.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Afferent Pathways
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Animals
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Arcuate Nucleus of Hypothalamus / cytology
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Arcuate Nucleus of Hypothalamus / physiology*
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Arcuate Nucleus of Hypothalamus / virology
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Brain / cytology
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Brain / physiology*
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Brain / virology
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Brain Mapping
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Chromosomes, Artificial, Bacterial
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Eating*
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Gene Expression
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Green Fluorescent Proteins
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Herpesvirus 1, Suid / genetics*
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Herpesvirus 1, Suid / physiology
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Hypothalamus / cytology
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Hypothalamus / physiology*
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Hypothalamus / virology
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Integrases / genetics
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Integrases / metabolism
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Neurons / metabolism*
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Neurons / virology
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Neuropeptide Y / genetics
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Neuropeptide Y / metabolism
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Receptors, Cell Surface*
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Receptors, Leptin
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Recombinant Fusion Proteins / metabolism
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Recombination, Genetic
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Viral Proteins*
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Virus Replication
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tau Proteins / genetics
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tau Proteins / metabolism
Substances
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Carrier Proteins
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Luminescent Proteins
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Neuropeptide Y
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Receptors, Cell Surface
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Receptors, Leptin
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Recombinant Fusion Proteins
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Viral Proteins
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tau Proteins
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Green Fluorescent Proteins
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Cre recombinase
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Integrases