Efficacy of surgical measures in preventing port-site recurrences in a porcine model

Surg Endosc. 2001 Feb;15(2):121-5. doi: 10.1007/s004640010069.

Abstract

Background: Port-site recurrences are serious complications of laparoscopy performed for cancer. Incidences reported in the literature vary between 0% and 21%, suggesting an influence of the surgeon.

Methods: The aim of this experimental, prospective, randomized, single-blind study was to investigate the influence that the quality surgery has on the incidence of port-site recurrences. After a 12-mmHg carbon dioxide (CO2) pneumoperitoneum was created, 10(7) human HeLa cell were injected into the peritoneal cavity of 18 pigs, creating a xenogeneic tumor. Laparoscopic sigmoid resections then were performed using four trocars and a transanal double-stapling technique. The following protective measures were applied in nine animals: trocar fixation, prevention of gas leaks, rinsing of instruments with povidone-iodine, minilaparotomy protection, rinsing of trocars before removal, peritoneal closure, and rinsing of all wounds with povidoneiodine. Surgeons and type of procedures were randomized. After 4 weeks, the animals were killed and all portsites excised. Blinded immunohistologic analysis with antihuman pancytokeratin antibody was performed.

Results: Tumor recurrence was present in 23 of 36 port sites (63.8%) in the control group, but only in 5 of 36 port sites (13.8%) in the group that received protective measures (p = 0.002; Fisher's exact test). No peritoneal carcinosis nor anastomotic recurrences were observed.

Conclusion: These results strongly suggest that the quality of surgical technique has an influence on the incidence of port-site recurrences. From now on, we propose to use these protective measures routinely in cancer laparoscopy.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • HeLa Cells
  • Humans
  • Immunohistochemistry
  • Laparoscopy / adverse effects*
  • Laparoscopy / methods
  • Neoplasm Recurrence, Local / prevention & control*
  • Neoplasm Seeding*
  • Primary Prevention / methods*
  • Probability
  • Reference Values
  • Sensitivity and Specificity
  • Sigmoid Neoplasms / pathology*
  • Sigmoid Neoplasms / surgery*
  • Statistics, Nonparametric
  • Swine