Abstract
We demonstrate that dendritic cells loaded in vitro with human immunodeficiency virus type 1 (HIV-1) protein-liposome complexes activate HLA class I-restricted anti-HIV-1 cytotoxic T-lymphocyte and gamma interferon (IFN-gamma) responses in autologous CD8+ T cells from late-stage HIV-1-infected patients on prolonged combination drug therapy. Interleukin-12 enhanced this effect through an interleukin-2- and IFN-gamma-mediated pathway. This suggests that dendritic cells from HIV-1-infected persons can be engineered to evoke stronger anti-HIV-1 CD8+ T-cell reactivity as a strategy to augment antiretroviral therapy.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anti-HIV Agents / therapeutic use
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Antiretroviral Therapy, Highly Active
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CD8-Positive T-Lymphocytes / immunology*
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CD8-Positive T-Lymphocytes / virology
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Cytotoxicity, Immunologic
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Dendritic Cells / immunology*
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HIV Infections / blood
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HIV Infections / drug therapy
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HIV Infections / immunology*
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HIV Infections / virology
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HIV-1 / immunology*
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Humans
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Interferon-gamma / biosynthesis
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Interleukin-12 / pharmacology
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Interleukin-2 / immunology
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Time Factors
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Viral Proteins / immunology*
Substances
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Anti-HIV Agents
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Interleukin-2
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Viral Proteins
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Interleukin-12
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Interferon-gamma