Abstract
A gamma camera imaging method was developed to detect dual gene transfer to adherent cells growing as monolayers in cell culture plates. Human cancer cells were infected with replication-incompetent adenoviral vectors encoding the human type 2 somatostatin receptor (Ad-hSSTr2) and/or herpes virus thymidine kinase (Ad-TK). The hSSTr2 and TK reporter proteins were detected by imaging internally bound (99m)Tc-P2045 peptide (Diatide, Inc.) and radioiodinated 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-5-iodouracil (FIAU), respectively. Following gene transfer, expression of hSSTr2 and TK were accurately imaged in vitro.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae
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Arabinofuranosyluracil / analogs & derivatives*
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Arabinofuranosyluracil / pharmacokinetics
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Carcinoma, Non-Small-Cell Lung
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Cell Adhesion
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Cell Line
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Gamma Cameras
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Gamma Rays
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Genetic Vectors
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Herpesviridae / genetics
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Humans
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Iodine Radioisotopes / pharmacokinetics
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Kidney
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Lung Neoplasms
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Radiopharmaceuticals
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Receptors, Somatostatin / genetics*
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Technetium / pharmacokinetics
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Thymidine Kinase / genetics*
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Transfection / methods*
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Tumor Cells, Cultured
Substances
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Iodine Radioisotopes
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Radiopharmaceuticals
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Receptors, Somatostatin
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Arabinofuranosyluracil
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fialuridine
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Technetium
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somatostatin receptor 2
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Thymidine Kinase