Two-hybrid dual bait system to discriminate specificity of protein interactions in small GTPases

Methods Enzymol. 2001:332:277-300. doi: 10.1016/s0076-6879(01)32210-3.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cloning, Molecular
  • DNA Primers / genetics
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Expression
  • Gene Library
  • Genetic Vectors
  • Humans
  • Monomeric GTP-Binding Proteins / genetics
  • Monomeric GTP-Binding Proteins / metabolism*
  • Plasmids / genetics
  • Protein Binding
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Transformation, Genetic
  • Two-Hybrid System Techniques*
  • cdc42 GTP-Binding Protein / genetics
  • cdc42 GTP-Binding Protein / metabolism

Substances

  • DNA Primers
  • Fungal Proteins
  • Recombinant Fusion Proteins
  • Monomeric GTP-Binding Proteins
  • cdc42 GTP-Binding Protein