Occult tumor cells in surgical specimens from cases of early cervical cancer treated by liposuction-assisted nerve-sparing radical hysterectomy

Int J Gynecol Cancer. 2001 Mar-Apr;11(2):159-63. doi: 10.1046/j.1525-1438.2001.011002159.x.

Abstract

Tumor recurrence in patients with cervical cancer after primary radical surgery with complete tumor resection (R0) may be caused by occult tumor cell deposits (OTCD) in potentially tumor-bearing pelvic tissue. New surgical techniques, like liposuction-assisted nerve-sparing radical abdominal hysterectomy (LANS-RH) might be performed to remove this tissue. In a preliminary study of 30 cases, treated with LANS-RH, the liposuction specimens were examined on hematoxylin & eosin (H&E)-stained step sections and at immunohistochemical levels, using the antibody cocktails MNF 116 and AE1/AE3 for cytokeratins to detect occult tumor cells. In nine cases microscopically tumor-free lymph nodes were detected in the liposuction material. One case presented lymphovascular space involvement in the capsular region of a node on H&E stained slides. Two additional cases showed tumor cell deposits in pelvic perinodal fatty tissue on immunohistochemical examination. We conclude that the LANS-RH technique is capable of resecting potentially tumor-bearing pelvic tissue. Careful histologic handling and immunohistochemical techniques may improve the detection of OTCD.

MeSH terms

  • Abdomen / surgery
  • Adult
  • Aged
  • Antibodies
  • Female
  • Humans
  • Hysterectomy / methods*
  • Immunohistochemistry
  • Keratins
  • Lipectomy*
  • Middle Aged
  • Neoplasm, Residual*
  • Neoplastic Cells, Circulating*
  • Pelvis
  • Sensitivity and Specificity
  • Uterine Cervical Neoplasms / surgery*

Substances

  • Antibodies
  • Keratins