Abstract
A genomic library of Candida tropicalis in a yeast multicopy plasmid has been screened for clones conferring salt tolerance upon transformation into S. cerevisiae. The best halotolerance clone contained an open reading frame encoding a predicted protein of 728 amino acids with homology to transcription factors of the heat-shock family. This novel gene was named HSR1 and is present in single copy in the C. tropicalis genome. Upon transformation into S. cerevisiae it increases the expression of ENA1, a major determinant of salt tolerance encoding a cation-extrusion pump. The sequence of CtHSR1 has been deposited in the EMBL data library under Accession No. AJ296093.
Copyright 2001 John Wiley & Sons, Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / biosynthesis
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Adenosine Triphosphatases / genetics
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Amino Acid Sequence
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Base Sequence
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Candida / genetics*
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Cation Transport Proteins*
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DNA-Binding Proteins / genetics
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Fungal Proteins / chemistry
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Fungal Proteins / genetics*
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Fungal Proteins / physiology
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Gene Library
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Heat-Shock Proteins / genetics
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Mediator Complex
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Molecular Sequence Data
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Nuclear Proteins / genetics*
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Nuclear Proteins / physiology
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Sodium Chloride / pharmacology
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Sodium-Potassium-Exchanging ATPase
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Transcription Factors / genetics*
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Transcription Factors / physiology
Substances
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Cation Transport Proteins
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DNA-Binding Proteins
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ENA1 protein, S cerevisiae
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Fungal Proteins
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HSF1 protein, S cerevisiae
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Heat-Shock Proteins
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Mediator Complex
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Nuclear Proteins
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PGD1 protein, S cerevisiae
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SFL1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Sodium Chloride
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Adenosine Triphosphatases
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Sodium-Potassium-Exchanging ATPase