Sequences in influenza A virus PB2 protein that determine productive infection for an avian influenza virus in mouse and human cell lines

Y Yao; L J Mingay; J W McCauley; W S Barclay

doi:10.1128/JVI.75.11.5410-5415.2001

Sequences in influenza A virus PB2 protein that determine productive infection for an avian influenza virus in mouse and human cell lines

J Virol. 2001 Jun;75(11):5410-5. doi: 10.1128/JVI.75.11.5410-5415.2001.

Authors

Y Yao¹, L J Mingay, J W McCauley, W S Barclay

Affiliation

¹ School of Animal and Microbial Sciences, University of Reading, Reading, Berkshire RG6 6AJ, United Kingdom.

Abstract

Reverse genetics was used to analyze the host range of two avian influenza viruses which differ in their ability to replicate in mouse and human cells in culture. Engineered viruses carrying sequences encoding amino acids 362 to 581 of PB2 from a host range variant productively infect mouse and human cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Genes, Viral
Humans
Influenza A virus / chemistry
Influenza A virus / genetics*
Influenza A virus / pathogenicity
Mice
RNA-Dependent RNA Polymerase
Sequence Analysis, Protein
Species Specificity
Transfection
Viral Proteins / genetics*

Substances

PB2 protein, Influenzavirus A
Viral Proteins
RNA-Dependent RNA Polymerase