Interferon-alpha induces transient suppressors of cytokine signalling expression in human T cells

Exp Clin Immunogenet. 2001;18(2):80-5. doi: 10.1159/000049186.

Abstract

The suppressors of cytokine signalling (SOCS) proteins comprise a newly identified family of negative feedback regulators of cytokine signalling. SOCS expression is differentially induced upon cytokine stimulation in different cell types. Here we show that interferon-alpha (IFNalpha) is a potent inducer of SOCS expression in human T cells, as high expression of CIS, SOCS-1, SOCS-2, and SOCS-3 was detectable after IFNalpha stimulation. After 4 h of stimulation, CIS, SOCS-1, and SOCS-3 expression had returned to baseline levels, whereas SOCS-2 expression had not declined. In contrast, after IL-2 induction neither CIS, SOCS-1, nor SOCS-2 expression levels declined after 6 h. In conclusion, we provide the first evidence that IFNalpha induces SOCS expression in human T cells. Moreover, we show that IFNalpha and IL-2 induce distinct patterns of expression kinetics, suggesting that dynamic changes in cytokine sensitivity might be mediated via induction of SOCS expression with different kinetics in T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD4-Positive T-Lymphocytes / drug effects
  • CD4-Positive T-Lymphocytes / immunology*
  • Carrier Proteins / genetics*
  • Cell Line
  • DNA-Binding Proteins*
  • Gene Expression / drug effects*
  • Humans
  • Interferon-alpha / immunology*
  • Interferon-alpha / pharmacology
  • Interleukin-2 / pharmacology
  • Intracellular Signaling Peptides and Proteins*
  • Proteins / genetics*
  • Repressor Proteins*
  • Signal Transduction*
  • Suppressor of Cytokine Signaling 1 Protein
  • Suppressor of Cytokine Signaling 3 Protein
  • Suppressor of Cytokine Signaling Proteins
  • Trans-Activators*
  • Transcription Factors*

Substances

  • Carrier Proteins
  • DNA-Binding Proteins
  • Interferon-alpha
  • Interleukin-2
  • Intracellular Signaling Peptides and Proteins
  • Proteins
  • Repressor Proteins
  • SOCS1 protein, human
  • SOCS2 protein, human
  • SOCS3 protein, human
  • Suppressor of Cytokine Signaling 1 Protein
  • Suppressor of Cytokine Signaling 3 Protein
  • Suppressor of Cytokine Signaling Proteins
  • Trans-Activators
  • Transcription Factors