Priming by DNA immunization augments protective efficacy of Mycobacterium bovis Bacille Calmette-Guerin against tuberculosis

Infect Immun. 2001 Jun;69(6):4174-6. doi: 10.1128/IAI.69.6.4174-4176.2001.

Abstract

Sequential immunization with mycobacterial antigen Ag85B-expressing DNA and Mycobacterium bovis bacille Calmette-Guerin (BCG) was more effective than BCG immunization in protecting against Mycobacterium tuberculosis infection. Depletion of the CD8(+) T cells in the immunized mice impaired protection in their spleens, indicating that this improved efficacy was partially mediated by CD8(+) T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyltransferases*
  • Animals
  • Antigens, Bacterial / administration & dosage
  • Antigens, Bacterial / immunology
  • Bacterial Proteins / administration & dosage
  • Bacterial Proteins / immunology*
  • Immunization, Secondary*
  • Mice
  • Mice, Inbred C57BL
  • Mycobacterium bovis / immunology*
  • Mycobacterium tuberculosis / immunology
  • Tuberculosis / immunology
  • Tuberculosis / prevention & control*
  • Vaccines, DNA / administration & dosage
  • Vaccines, DNA / immunology*

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • Vaccines, DNA
  • Acyltransferases
  • antigen 85B, Mycobacterium tuberculosis