The respiratory complex I in yeast: isolation of a gene NUO51 coding for the nucleotide-binding subunit of NADH:ubiquinone oxidoreductase from the obligately aerobic yeast Yarrowia lipolytica

Folia Microbiol (Praha). 2000;45(5):429-33. doi: 10.1007/BF02817616.

Abstract

We have isolated a gene NUO51 coding for a homologue of the nucleotide-binding subunit of mitochondrial respiratory chain linked NADH:ubiquinone oxidoreductase from the obligately aerobic yeast Yarrowia lipolytica. DNA sequencing revealed a 1464 bp open reading frame encoding a protein with predicted molar mass of about 53.7 kDa. The sequence is highly conserved with its counterparts from filamentous fungi and represents the first yeast homologue of the NADH-binding subunit (51 kDa) of the respiratory complex 1. In addition, PFGE and Southern hybridization analysis indicate that NUO51 is a single copy gene in the genome of Y. lipolytica. The expression of NUO51 by Northern blot analysis was also examined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blotting, Southern
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • DNA, Complementary / genetics
  • DNA, Fungal / analysis
  • DNA, Fungal / genetics
  • Electron Transport Complex I
  • Electrophoresis, Gel, Pulsed-Field
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Molecular Sequence Data
  • NADH, NADPH Oxidoreductases / chemistry*
  • NADH, NADPH Oxidoreductases / genetics*
  • NADH, NADPH Oxidoreductases / metabolism
  • Nucleotides / metabolism
  • Saccharomycetales / enzymology*
  • Saccharomycetales / genetics
  • Saccharomycetales / growth & development
  • Sequence Analysis, DNA

Substances

  • Carrier Proteins
  • DNA, Complementary
  • DNA, Fungal
  • Fungal Proteins
  • Nucleotides
  • NADH, NADPH Oxidoreductases
  • NUBM protein, Yarrowia lipolytica
  • Electron Transport Complex I

Associated data

  • GENBANK/AF146339
  • GENBANK/AF197536