Abstract
Peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1) is highly expressed in brown adipose tissue (BAT) and plays an important role in adaptive thermogenesis. The aim of this study was to assess the acute effect of a beta(3)-adrenergic agonist (Trecadrine) and leptin on the expression of PGC-1 and PPARgamma2 mRNA in BAT. Trecadrine produced a marked increase (4.5-fold) in PGC-1 mRNA compared to controls (P<0.001) without changes in PPARgamma2 mRNA, whereas leptin administration did not alter either PGC-1 or PPARgamma2 expression. These results show that selective stimulation of the beta(3)-adrenoceptor rapidly upregulates the expression of PGC-1 in brown adipocytes without a concomitant increase in PPARgamma2. Moreover, our results show that PGC-1 and PPARgamma2 expression in BAT seems not to be acutely regulated by leptin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue, Brown / drug effects*
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Adipose Tissue, Brown / metabolism
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Adrenergic beta-Agonists / pharmacology*
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Animals
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Benzyl Alcohols / pharmacology*
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Body Temperature / drug effects
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Carrier Proteins / genetics
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Fasting
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Fatty Acids, Nonesterified / blood
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Ion Channels
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Leptin / pharmacology*
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Male
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Membrane Proteins / genetics
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Mitochondrial Proteins
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Oxygen Consumption / drug effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Rats, Wistar
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Receptors, Cytoplasmic and Nuclear / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Transcription Factors / genetics*
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Uncoupling Protein 1
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Up-Regulation / drug effects*
Substances
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Adrenergic beta-Agonists
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Benzyl Alcohols
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Carrier Proteins
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Fatty Acids, Nonesterified
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Ion Channels
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Leptin
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Membrane Proteins
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Mitochondrial Proteins
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RNA, Messenger
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Receptors, Cytoplasmic and Nuclear
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Transcription Factors
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Uncoupling Protein 1
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peroxisome-proliferator-activated receptor-gamma coactivator-1
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trecadrine