Assaying mitochondrial respiratory complex activity in mitochondria isolated from human cells and tissues

Methods Cell Biol. 2001:65:97-117. doi: 10.1016/s0091-679x(01)65006-4.
No abstract available

MeSH terms

  • Animals
  • Biological Assay / methods
  • Cell Fractionation
  • Cells, Cultured
  • Detergents / pharmacology
  • Electron Transport / physiology
  • Electron Transport Complex I
  • Electron Transport Complex II
  • Electron Transport Complex III / metabolism*
  • Electron Transport Complex IV / metabolism*
  • Fibroblasts / chemistry
  • Fibroblasts / enzymology
  • Fibroblasts / metabolism
  • Glucosides / pharmacology
  • Humans
  • Intracellular Membranes / metabolism
  • Mitochondria / chemistry
  • Mitochondria / enzymology*
  • Mitochondria / metabolism
  • Mitochondria, Muscle / chemistry
  • Mitochondria, Muscle / enzymology
  • Mitochondria, Muscle / metabolism
  • Multienzyme Complexes / metabolism*
  • Muscle, Skeletal / chemistry
  • Muscle, Skeletal / cytology
  • Muscle, Skeletal / enzymology*
  • NADH, NADPH Oxidoreductases / metabolism*
  • Oxidation-Reduction
  • Oxidoreductases / metabolism*
  • Protein Subunits
  • Proton Pumps / metabolism
  • Spectrophotometry / methods
  • Succinate Dehydrogenase / metabolism*
  • Tissue Extracts / metabolism

Substances

  • Detergents
  • Glucosides
  • Multienzyme Complexes
  • Protein Subunits
  • Proton Pumps
  • Tissue Extracts
  • dodecyl maltoside
  • Oxidoreductases
  • Electron Transport Complex II
  • Succinate Dehydrogenase
  • NADH, NADPH Oxidoreductases
  • Electron Transport Complex IV
  • Electron Transport Complex I
  • Electron Transport Complex III