Abstract
Islet-brain1/JNK-interacting protein-1 (IB1/JIP-1) is a scaffold protein that organizes the JNK, MKK7, and MLK1 to allow signaling specificity. Targeted disruption of the gene MAPK8IP1 encoding IB1/JIP-1 in mice led to embryonic death prior to blastocyst implantation. In culture, no IB1/JIP-1(-/-) embryos were identified indicating that accelerated cell death occurred during the first cell cycles. IB1/JIP-1 expression was detected in unfertilized oocytes, in spermatozoa, and in different stages of embryo development. Thus, despite the maternal and paternal transmission of the IB1/JIP-1 protein, early transcription of the MAPK8IP1 gene is required for the survival of the fertilized oocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Animals
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Apoptosis
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Blastocyst / metabolism
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Blotting, Western
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Carrier Proteins / physiology*
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Cell Death
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Cell Division
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Cell Survival
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DNA, Complementary / metabolism
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Embryo, Mammalian / metabolism
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Female
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Heterozygote
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MAP Kinase Kinase 7
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MAP Kinase Kinase Kinases / metabolism
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Male
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Mice
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Mice, Inbred C57BL
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Microscopy, Fluorescence
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Mitogen-Activated Protein Kinase 8
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Mitogen-Activated Protein Kinase Kinases / metabolism
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Mitogen-Activated Protein Kinases / metabolism
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Nuclear Proteins / physiology*
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Rats
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Reverse Transcriptase Polymerase Chain Reaction
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Signal Transduction
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Spermatozoa / metabolism
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Testis / metabolism
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Trans-Activators / physiology*
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Transfection
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Zygote / metabolism
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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DNA, Complementary
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MAPK8IP1 protein, human
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Mapk8ip protein, mouse
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Mapk8ip1 protein, rat
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Nuclear Proteins
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Trans-Activators
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Mitogen-Activated Protein Kinase 8
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Mitogen-Activated Protein Kinases
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MAP Kinase Kinase Kinases
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Map3k9 protein, mouse
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Map3k9 protein, rat
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MAP Kinase Kinase 7
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MAP2K7 protein, human
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Map2k7 protein, mouse
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Mitogen-Activated Protein Kinase Kinases