Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine

Science. 2001 Apr 6;292(5514):69-74. doi: 10.1126/science.1058915.

Abstract

Heterologous prime/boost regimens have the potential for raising high levels of immune responses. Here we report that DNA priming followed by a recombinant modified vaccinia Ankara (rMVA) booster controlled a highly pathogenic immunodeficiency virus challenge in a rhesus macaque model. Both the DNA and rMVA components of the vaccine expressed multiple immunodeficiency virus proteins. Two DNA inoculations at 0 and 8 weeks and a single rMVA booster at 24 weeks effectively controlled an intrarectal challenge administered 7 months after the booster. These findings provide hope that a relatively simple multiprotein DNA/MVA vaccine can help to control the acquired immune deficiency syndrome epidemic.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • AIDS Vaccines / administration & dosage
  • AIDS Vaccines / immunology*
  • Acquired Immunodeficiency Syndrome / immunology
  • Acquired Immunodeficiency Syndrome / prevention & control*
  • Acquired Immunodeficiency Syndrome / virology
  • Animals
  • Antibodies, Viral / blood
  • Antibodies, Viral / immunology
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / immunology
  • Germinal Center / immunology
  • HIV Antibodies / blood
  • HIV Antibodies / immunology
  • HIV-1 / genetics
  • HIV-1 / immunology
  • HIV-1 / physiology
  • Immunity, Mucosal
  • Immunization, Secondary
  • Immunologic Memory
  • Interferon-gamma / biosynthesis
  • Lymph Nodes / immunology
  • Macaca mulatta
  • SAIDS Vaccines / administration & dosage
  • SAIDS Vaccines / immunology
  • Simian Acquired Immunodeficiency Syndrome / immunology
  • Simian Acquired Immunodeficiency Syndrome / prevention & control
  • Simian Acquired Immunodeficiency Syndrome / virology
  • Simian Immunodeficiency Virus / genetics
  • Simian Immunodeficiency Virus / immunology
  • Simian Immunodeficiency Virus / physiology
  • T-Lymphocytes / immunology
  • Vaccines, DNA / administration & dosage
  • Vaccines, DNA / immunology*
  • Vaccines, Synthetic / administration & dosage
  • Vaccines, Synthetic / immunology
  • Vaccinia virus / immunology
  • Viral Load

Substances

  • AIDS Vaccines
  • Antibodies, Viral
  • HIV Antibodies
  • SAIDS Vaccines
  • Vaccines, DNA
  • Vaccines, Synthetic
  • Interferon-gamma