Objective: Investigate the sensitivity and accuracy of the denaturing high performance liquid chromatography(DHPLC) technique for the detection of single nucleotide polymorphism(SNP).
Methods: Forty-one samples were detected by both DHPLC and direct sequencing.
Results: The comparison demonstrated that DHPLC detected all heterozygous sequences found by direct sequencing. No false-positive signals were seen in the cases of homozygous sequences. Furthermore, no false-negative results were ever obtained with heterozygous mutations or polymorphisms, or both.
Conclusion: DHPLC is a potent method for SNP identification especially SNP typing in large scale screening.