Abstract
Resistance to teicoplanin in clinical isolates of Staphylococcus aureus has increased in frequency. Detection is difficult due to heterogeneous phenotypes of these strains. Two mutations in pbp4 have recently been identified in glycopeptide resistant laboratory strains. We investigated to determine if these mutations could be used to screen clinical isolates for teicoplanin resistance. Of 41 clinical isolates screened, none contained the mutations in pbp4 indicating these mutations cannot be used as a molecular diagnostic tool for glycopeptide resistance.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins*
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Carrier Proteins / genetics*
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DNA Mutational Analysis
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DNA, Bacterial / analysis
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Drug Resistance, Microbial
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Hexosyltransferases*
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Humans
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Microbial Sensitivity Tests
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Muramoylpentapeptide Carboxypeptidase / genetics*
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Penicillin-Binding Proteins
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Peptidyl Transferases*
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Phenotype
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Polymerase Chain Reaction
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / genetics
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Staphylococcus aureus / isolation & purification
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Teicoplanin / pharmacology*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carrier Proteins
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DNA, Bacterial
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Penicillin-Binding Proteins
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Teicoplanin
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Peptidyl Transferases
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Hexosyltransferases
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Muramoylpentapeptide Carboxypeptidase