The identification of microorganisms by fluorescence in situ hybridisation

R Amann; B M Fuchs; S Behrens

doi:10.1016/s0958-1669(00)00204-4

The identification of microorganisms by fluorescence in situ hybridisation

Curr Opin Biotechnol. 2001 Jun;12(3):231-6. doi: 10.1016/s0958-1669(00)00204-4.

Authors

R Amann¹, B M Fuchs, S Behrens

Affiliation

¹ Molecular Ecology Group, Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D-28359, Bremen, Germany. [email protected]

PMID: 11404099
DOI: 10.1016/s0958-1669(00)00204-4

Abstract

Fluorescence in situ hybridisation (FISH) with rRNA-targeted oligonucleotide probes facilitates the rapid and specific identification of individual microbial cells in their natural environments. Over the past year there have been a number of methodological developments in this area and new applications of FISH in microbial ecology and biotechnology have been reported.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Bacteria / genetics
Bacteria / isolation & purification*
Environmental Microbiology
Geologic Sediments / chemistry
Geologic Sediments / microbiology
In Situ Hybridization, Fluorescence / methods*
Molecular Probes / chemistry
RNA, Ribosomal, 16S / genetics
RNA, Ribosomal, 16S / physiology*
Sensitivity and Specificity
Sewage / microbiology
Symbiosis

Substances

Molecular Probes
RNA, Ribosomal, 16S
Sewage