Influence of strain and trisodium phosphate concentration on growth parameters of Listeria monocytogenes in vitro

Lett Appl Microbiol. 2001 Jun;32(6):428-32. doi: 10.1046/j.1472-765x.2001.00936.x.

Abstract

Aims: The present study was conducted to determine the influence of strain and trisodium phosphate (TSP) concentration in the growth of Listeria monocytogenes in vitro.

Methods and results: Three strains (ATCC 11916, 64d, isolated from chicken meat, and M2-5b, a clinical animal isolate) were inoculated in broth with 0, 0.5, 1 and 1.5% (w/v) of TSP. The shortest lag phase and highest maximum rate of growth (mu) were obtained in the presence of 0.5% TSP. In contrast, the highest lag phase and lowest mu were obtained with 1.5% TSP. For each TSP concentration, significant differences (P < 0.05) in lag phase and mu of the three L. monocytogenes strains were observed.

Conclusion: The behaviour of L. monocytogenes is significantly influenced by both the origin of the strain and the salt concentration.

Significance and impact of the study: Our results indicate the importance of choosing an adequate TSP concentration for the decontamination of foods, because low concentrations could favour the growth of L. monocytogenes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cathartics / pharmacology
  • Culture Media
  • Food Contamination
  • Food Microbiology
  • Hydrogen-Ion Concentration
  • Listeria monocytogenes / classification
  • Listeria monocytogenes / drug effects*
  • Listeria monocytogenes / growth & development
  • Osmolar Concentration
  • Phosphates / pharmacology*

Substances

  • Cathartics
  • Culture Media
  • Phosphates
  • sodium phosphate