Nested case-control study in a serological survey to evaluate the effectiveness of a Chagas disease control programme in Brazil

Bull World Health Organ. 2001;79(5):409-14.

Abstract

Objective: To identify risk factors associated with Trypanosoma cruzi infections in areas under surveillance in the State of Minas Gerais, Brazil.

Methods: A model using a nested case-control design incorporated within a serological survey of schoolchildren which was employed to evaluate the effectiveness of the Chagas disease control programme.

Findings: In a sample of 40,374 schoolchildren (aged 7-14 years) surveyed, 16 children tested positive for T. cruzi antibody (by indirect immunofluorescence and indirect haemagglutination). In the case-control study, each case was randomly matched to three seronegative controls (classroom and age +/- 1 year). Compared to controls, T. cruzi-seropositive children were more likely to have a seropositive mother (odds ratio (OR) = 6.8; 95% confidence interval (CI) = 0.71-63.9) or a seropositive family member (OR = 8.6; 95% CI = 1.0-75.5).

Conclusion: Use of the nested case-control model in a sero-epidemiological survey to evaluate risk factors for T. cruzi transmission was adequate for assessing the effectiveness of a Chagas disease control programme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Brazil / epidemiology
  • Case-Control Studies
  • Chagas Disease / diagnosis
  • Chagas Disease / epidemiology
  • Chagas Disease / prevention & control*
  • Child
  • Communicable Disease Control*
  • Female
  • Humans
  • Male
  • Population Surveillance
  • Program Evaluation / methods*
  • Risk Factors