[Scanning electron microscopy and light microscopy under polarized light of a submandibular salivary gland calculus]

Minerva Stomatol. 2001 May;50(5):173-80.
[Article in Italian]

Abstract

Background: The salivary calculus is an ancient pathologic condition, called sublingual stone by Hyppocrates. It is well-defined from a anatomo-pathologic, diagnostic and topographic viewpoint, though some pathogenesis-related doubts still exist. This work aims at relating the micro-morpho-structural aspect of a salivary calculus of remarkable dimensions with the current calculogenetic hypotheses.

Methods: A 1.1 g salivary calculus removed from the initial section of Wrthon s duct in the right-hand submandibular gland of a 42 year old male has been studied. Following the fixation in 4% buffered formalin and the inclusion in resin, it was serially sectioned in 15-20 micron slices. Some sections were coloured with toluidine blue O and acid fuchsin. The exeresis of the calculus was carried out intraorally, with marsupialization under local anesthesia. The analysis was performed using a SEM with backscattered electrons and a polarised and transmitted light microscope.

Results: The investigations have outlined the presence of various organic cores and a concentric, stratiform architecture interrupted by radial elements.

Conclusions: The formation of this calculus may be due to mixed secondary lithiasis resulting from the formation of colloids and crystalloids.

Publication types

  • Case Reports
  • English Abstract

MeSH terms

  • Adult
  • Humans
  • Male
  • Microscopy, Electron, Scanning
  • Microscopy, Polarization
  • Salivary Gland Calculi / ultrastructure*
  • Submandibular Gland Diseases / pathology*